Frontiers in Peptidome Research:
Methods for Analysis and Applications

Date and site

Date : January 28 (Wed.) --29(Thur.), 2004
Site : Science Hall, Senri Life Science Center 5F
Address : Shinsenri-Higashimachi, Toyonaka, Osaka 560-0082, Japan
Phone : +81-6-6873-2020
Fax : +81-6-6873-2011

Date & Time: Wednesday, January 28, 2004. 18:30-20:00

Naoto Minamino, National Cardiovascular Center
Kenji Kangawa, National Cardiovascular Center
Toshifumi Takao, Institute for Protein Research, Osaka University

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Program

January 28, Wednesday

9:00-9:05
Opening remarks
Takeshi Oishi, Research Promotion Committee of the Peptidome Project

9:05-10:45
Session 1: Peptidomics in the comprehensive analysis of endogenous peptides
Chair: P. Andren and N. Minamino


1. Peptidome project: Analysis of peptides in pig and mouse brain for the Peptidome database
Naoto Minamino, Junko Isoyama-Tanaka, Takahiro Kihara, Matsui Yasuko, Hiromiki Kuwahara
Department of Pharmacology, National Cardiovascular Center Research Institute, Osaka, Japan

2. Peptidome: Data acquisition, storage and presentation
Masaharu Isoyama
Department of Information, Protein Research Foundation, Osaka, Japan

3. Peptidomics-based discovery of peptide tumor markers
Kazuki Sasaki
Growth Factor Division, National Cancer Center Research institute, Tokyo, Japan

10:45-11:00
Coffee Break

11:00-12:10
4. Peptidomics-based discovery of novel neuropeptides
Per E. Andren
Medical Mass Spectrometry Research Laboratory, Uppsala University, Sweden

5. Systematic identification of peptide signaling molecules in Hydra --Recent progress in functional analysis of neuropeptides--
Toshitaka Fujisawa
Department of Developmental Genetics, National Institute of Genetics, Mishima, Japan

12:10-13:10
Lunch

13:10-15:20
Session 2: Peptidomics and screening of new peptides
Chair: L. Schoofs and T. Haga


6. Peptidomics in insects: Peptide identification in the post-genome
Liliane Schoofs and Geert Baggerman
Laboratory for Developmental Physiology, Genomics and Proteomics
Katholieke Universiteit Leuven, Belgium

7. Structure and action of the cardiovascular regulatory neuropeptides in Aplysia
Yasuo Furukawa, Kosei Sasaki, Fumihiko Morishita, Osamu Matsushima
Department of Biological Science, Faculty of Science, Hiroshima University, Hiroshima, Japan

8. Identification of novel RFamide peptide genes by utilizing databases
Shuji Hinuma
Discovery Research Laboratories, Pharmaceutical Research Division, Takeda Chemical Industries, Tsukuba, Japan

9. Receptor-Galpha fusion proteins as a peptide-screening system
Tatsuya Haga, Shigeki Takeda, Hinako Suga, Naoto Minamino
Institute for Biomolecular Science, Gakushuin University, Tokyo, Japan

15:20-15:35
Coffee break

15:35-18:00
Session 3: Peptidomics and new biologically active peptides
Chair: H. Vaudry and N. Nakazato

10. Peptidomics from amphibian peptides to mammalian peptides
Hubert Vaudry, Cynthia Dujardin, Herve Tostivint, Jerome Leprince, Marie-Christine Tonon, J. Michael Conlon, Youssef Anouar, Isabelle Lihrmann, Nicolas Chartrel
Laboratory of Cellular and Molecular Neuroendocrinology, INSERM, University of Rouen, France


11. Identification and functional characterization of bioactive peptides from amphibian and reptile
Tai Kubo, Suzuko Kobayashi, Gandhi R. Baptista, Xianyu Yang, Hidetoshi Inagaki, Yuka Nakajima, Mami Takeda, Hikaru Urano, Tadashi Kimura, Alvaro Prieto-da-SilvaATetsuo Yamane
Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan

12. Calcitonin receptor-stimulating peptides: new members of the CGRP family with unique biological activity
Takeshi Katafuchi, Naoto Minamino
LDEBIS and Department of Pharmacology, National Cardiovascular Center Research Institute, Osaka, Japan

13. Ghrelin, a new regulator in the endocrine, central nervous and cardiovascular systems
Kenji Kangawa, Hiroshi Hosoda, Masayasu Kojima
Department of Biochemistry, National Cardiovascular Center Research Institute, Osaka, Japan

14. Feeding regulation by recently discovered peptides
Masamitsu Nakazato, Yukari Date, Naoto Minamino, Kenji Kangawa
Third Department of Internal Medicine, Miyazaki Medical College, University of Miyazaki, Miyazaki, Japan


18:30-20:00
Mixer party

January 29, Thursday

9:00-10:30
Session 4: Peptidomics and peptide conformation
Chair: M. Ishiguro and H. Mizushima

15. Integration of peptide database to multiple databases, and touch and feel system for peptide representation
Hiroshi Mizushima, Hiroshi Nagata, Hiroshi Tanaka
Cancer Information Division, National Cancer Center Research Institute, Tokyo, Japan

16. Studies on receptor-bound peptide conformation
Masaji Ishiguro, Kenji Sugase, Takahiro Hirano
Suntory Institute for Bioorganic Research, Osaka, Japan

17. Free-energy landscape of peptides and conformational classification
Junichi Higo
School of Life Science, Tokyo University of Pharmacy and Life Science, Tokyo, Japan

10:30-10:45
Coffee break

10:45-11:55
Session 5: Proteomics and emerging technology I
Chair: M. Kalkum and T. Takao

18. Mass spectrometry for peptidomic and proteomic analysis
Toshifumi Takao, Yoshinori Satomi, Jorge Fernandez-de-Cossio, Masami Matsubae
Research Center for Structural and Functional Proteomics, Institute for Protein Research, Osaka University, Osaka, Japan

19. Hypothesis-driven proteomics
Markus Kalkum
Beckman Research Institute of the City of Hope, California, USA

11:55-13:00
Lunch

13:00-14:15
Session 6. Proteomics and emerging technology II
Chair: W. Blackstock and T. Takao


20. New tools for proteome analysis
Osamu Nishimura
Life Science Laboratory/Proteomics Research Center, Shimadzu Corporation, Kyoto, and Research Center for Structural and Functional Proteomics, Institute for Protein Research, Osaka University, Osaka, Japan

21. Design and performance of a new hybrid LC-QIT-TOF mass spectrometer
Kozo Miseki, Jun-ichi Taniguchi, Eizo Kawatoh, Shin-ichi Yamaguchi, Hiroto Itoi, Jun-ichi Koide, Takami Yamada, Junko Iida, Shaun Bilsborough
Analytical and measuring Instrument Division, Shimadzu Corporation, Kyoto, Japan

22. Atmospheric pressure MALDI-orthogonal acceleration TOFMS (APMALDI-oaTOFMS): a new tool for proteomics and peptidomics research
Jun Tamura, Tetsuichiro Morita, Kenji Nagatomo, Phillip Tan
Analytical Instruments Division, JEOL Ltd., Tokyo, Japan

23. Data mining and database systems for large-scale proteome analyses
Takeshi Sakamoto
Life Science Group, Hitachi, Ltd., Saitama, Japan

14:15-14:30
Coffee break

14:30-16:50
Session 7: Proteomics and Proteome
Chair: Y.K. Paik and T. Nishimura

24. Cancer proteomics
Young-Ki Paik
Yonsei Proteome Research Center, Department of Biochemistry, Yonsei University
Seoul, Korea

25. A new strategy of large-scale protein profiling in clinical proteomics
Toshihide Nishimura
The Clinical Proteome Center, Tokyo Medical University, Tokyo, Japan

26. The strategy in proteomics and proteome informatics in TMIG
Toshifusa Toda, Hiraku Morisawa
Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan

27. Building a cell map: Experimental approaches to pathway expansion
Walter Blackstock
Protein Partners, United Kingdom, and Tokyo Medical University, Japan

16:50-16:55
Closing remarks
K. Kangawa, National Cardiovascular Center Research Institute